FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19918540
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA99900.09.91818494-95
AGCGTCG153700.09.49196494-95
GTCGTGT114800.09.37600694-95
GCGTCGT134700.09.29618294-95
CGTCGTG126100.09.19530994-95
CGTGTAG99500.08.54912394-95
ATCGGAA352300.08.39014294-95
GAGCGTC189550.08.37363994-95
GATCGGA376300.08.08234494-95
AGAGCGT208250.07.975423394-95
TCGGAAG345150.07.903065794-95
AAGAGCG241650.07.767869594-95
TAGATCG124100.07.658607594-95
AGATCGG421350.07.65805394-95
CGGAAGA347750.07.29052694-95
AAGATCG136950.06.003101394-95
CAGATCG153900.05.99038494-95
GTGTAGG134000.05.94020794-95
GAGATCG162000.05.60286294-95
GAAGAGC440250.05.143457494-95
Produced by FastQC (version 0.11.5)