FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21630195
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT154100.010.45428394-95
AGCGTCG175350.010.29852494-95
CGTCGTG139050.09.68900694-95
TCGTGTA114550.09.64550694-95
GTCGTGT128800.09.18714794-95
CGTGTAG110400.08.84585794-95
GAGCGTC217100.08.75583194-95
ATCGGAA419650.08.72534594-95
GATCGGA450700.08.451097594-95
AGAGCGT242000.08.38512794-95
AAGAGCG281450.08.31576394-95
TCGGAAG405350.08.23008194-95
AGATCGG508150.08.0941794-95
TAGATCG153950.08.07214894-95
CGGAAGA394600.07.50288294-95
AAGATCG166950.06.418850494-95
GAGATCG192300.06.375842694-95
GTGTAGG147450.06.36530994-95
CAGATCG169350.06.299822394-95
GTAGGGA138100.05.643484694-95
Produced by FastQC (version 0.11.5)