FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22449309
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG124950.010.05431394-95
AGCGTCG152250.09.85808994-95
TCGTGTA97450.09.28487694-95
GCGTCGT132250.09.19405894-95
GTCGTGT114700.09.1514994-95
ATCGGAA393300.09.10563894-95
GAGCGTC188000.09.08247194-95
AGATCGG482950.08.81188594-95
GATCGGA427100.08.8020694-95
CGGAAGA374350.08.66574494-95
TAGATCG153150.08.51311194-95
CGTGTAG99300.08.49008694-95
TCGGAAG373450.08.432260594-95
AAGAGCG256950.08.38284994-95
AGAGCGT211750.08.086205594-95
CAGATCG158800.06.95401294-95
GAGATCG185100.06.77423894-95
AAGATCG167100.06.70808494-95
GTGTAGG140050.05.56190794-95
TCGGAAA56600.05.412604394-95
Produced by FastQC (version 0.11.5)