FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22198562
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA92950.09.24974994-95
CGTCGTG118100.09.04968494-95
CGTGTAG95300.08.97181694-95
GCGTCGT126450.08.95922394-95
AGCGTCG142200.08.83541394-95
GATCGGA373050.08.70306594-95
AGATCGG421150.08.59446794-95
GTCGTGT109050.08.55928894-95
ATCGGAA342200.08.328694-95
AGAGCGT198400.08.18813994-95
AAGAGCG236650.08.05897594-95
GAGCGTC182050.07.918971594-95
TCGGAAG341600.07.80091994-95
TAGATCG129700.07.78251194-95
CGGAAGA352550.07.504733694-95
CAGATCG156200.06.76627794-95
AAGATCG145600.06.084406494-95
GAGATCG176900.05.558316794-95
GTGTAGG136000.05.51847394-95
GAAGAGC463650.05.07125894-95
Produced by FastQC (version 0.11.5)