FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22151732
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC128900.09.08115394-95
GATCGGA420300.09.06133194-95
ATCGGAA389750.08.9552694-95
AGATCGG473600.08.59302694-95
TCGGAAG379750.08.32199294-95
CGTCTGA100450.08.2730494-95
CGGAAGA374350.07.902913694-95
CACACGT168500.07.891055694-95
TAGATCG150350.07.88032594-95
GCACACG194000.07.722797494-95
CACGTCT140500.07.283631394-95
ACGTCTG120350.07.26020294-95
AAGATCG158300.06.62961694-95
CAGATCG165200.06.36708694-95
GAGATCG176150.05.957809494-95
AGCACAC290200.05.88272494-95
AGAGCAC333000.05.81825894-95
GAAGAGC475850.05.46874394-95
GAGCACA333050.05.2613194-95
AAGAGCA443950.05.214558694-95
Produced by FastQC (version 0.11.5)