FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24872845
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT102700.09.97064394-95
AGCGTCG119900.09.93510394-95
GATCGGA311900.09.7501894-95
CGTCGTG100450.09.68171894-95
ATCGGAA290950.09.45300694-95
GTCGTGT95950.08.9023394-95
AGATCGG354650.08.814280594-95
GAGCGTC156800.08.61437694-95
AGAGCGT175050.08.59813694-95
TCGTGTA85000.08.23304694-95
TCGGAAG289400.08.12565194-95
AAGAGCG206950.08.11817494-95
TAGATCG112250.08.04509794-95
CGTGTAG86950.07.427022594-95
CGGAAGA301600.07.242473694-95
AAGATCG132800.06.45144194-95
CAGATCG142550.05.919938694-95
CGATACG8403.5579047E-55.51343990-91
GAGATCG153650.05.22435494-95
TAACGCG6500.0077778435.145653794-95
Produced by FastQC (version 0.11.5)