FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21979443
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA83700.09.9093494-95
GTCGTGT94750.09.50615294-95
CGTCGTG101000.09.15320194-95
GCGTCGT106250.09.08117194-95
ATCGGAA311250.08.84950394-95
AGCGTCG124600.08.79280994-95
GATCGGA336200.08.73706394-95
AGATCGG382450.08.52558594-95
TAGATCG120050.08.43319694-95
TCGGAAG312250.08.38733394-95
AAGAGCG209650.08.37711294-95
GAGCGTC157800.08.32842594-95
AGAGCGT174550.08.27805894-95
CGTGTAG84150.08.04888194-95
CGGAAGA313400.07.80299294-95
AAGATCG141750.06.84040694-95
CAGATCG144450.06.317691394-95
GAGATCG167300.05.24173794-95
GTGTAGG125950.05.207816694-95
GAAGAGC416850.05.199478694-95
Produced by FastQC (version 0.11.5)