FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23313101
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA113400.09.89221194-95
AGCGTCG175850.09.63632994-95
GCGTCGT156500.09.53695694-95
GTCGTGT133300.09.46735994-95
CGTCGTG145400.09.30062994-95
AGAGCGT247150.09.09693294-95
ATCGGAA443950.09.09008894-95
CGTGTAG116150.09.02368394-95
GATCGGA480350.08.95045894-95
GAGCGTC221900.08.94321494-95
AAGAGCG288950.08.84199494-95
AGATCGG546100.08.69971794-95
TAGATCG159500.08.58274694-95
TCGGAAG432800.08.2424894-95
CGGAAGA429950.07.556401394-95
CAGATCG200100.07.185760594-95
AAGATCG179300.06.62756894-95
GAGATCG211400.06.23953894-95
GTGTAGG153200.05.75545494-95
GAAGAGC546850.05.61511694-95
Produced by FastQC (version 0.11.5)