FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18460307
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT106300.09.584578594-95
CGTCGTG99500.09.57128594-95
TCGTGTA79000.09.40949394-95
AGCGTCG120200.09.38508394-95
GTCGTGT92150.08.68527494-95
GATCGGA307100.08.39844794-95
ATCGGAA286150.08.31616294-95
GAGCGTC151800.08.260591594-95
TAGATCG113300.07.965309694-95
AGATCGG344350.07.95203494-95
AAGAGCG195150.07.84946694-95
AGAGCGT166350.07.823601794-95
CGTGTAG82600.07.820559594-95
CGGAAGA281700.07.49486794-95
TCGGAAG276000.07.22801794-95
CAGATCG128150.06.782838394-95
AAGATCG109500.06.202987794-95
GAGATCG134400.05.9549794-95
GTGTAGG112000.05.322365394-95
GAAGAGC370950.05.10900794-95
Produced by FastQC (version 0.11.5)