FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20819456
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG128050.010.0521794-95
TCGTGTA88000.09.55346194-95
GTCGTGT97950.09.40735294-95
ATCGGAA326850.09.2640794-95
GAGCGTC161800.09.24702894-95
CGTCGTG103650.09.23370294-95
GATCGGA356500.09.13975994-95
GCGTCGT109750.08.74212594-95
CGTGTAG89200.08.73271294-95
AGATCGG400900.08.66659394-95
TAGATCG126600.08.62907594-95
AAGAGCG213250.08.50833294-95
AGAGCGT179250.08.26733694-95
TCGGAAG313000.08.16409594-95
CGGAAGA318500.07.613010494-95
AAGATCG140550.06.70810494-95
GAGATCG154100.06.549775694-95
CAGATCG148950.06.47329994-95
GTGTAGG126450.05.46530494-95
GAAGAGC417500.05.25031594-95
Produced by FastQC (version 0.11.5)