FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20741325
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG130700.09.44870194-95
GTCGTGT101700.09.107264594-95
CGTCGTG107950.08.953979594-95
ATCGGAA328050.08.94793494-95
TCGTGTA88800.08.82562294-95
GCGTCGT112800.08.80058594-95
TAGATCG122350.08.5212894-95
AAGAGCG218400.08.36215194-95
GAGCGTC167600.08.34613994-95
GATCGGA352550.08.33284594-95
CGTGTAG87450.08.14715194-95
TCGGAAG315850.07.947644794-95
AGAGCGT182200.07.898936394-95
AGATCGG394150.07.875141694-95
CGGAAGA324700.07.643254894-95
CAGATCG142550.06.214210594-95
AAGATCG141150.05.704087792-93
GAGATCG153250.05.547877394-95
GTGTAGG127400.05.44324394-95
GAAGAGC424500.05.342813594-95
Produced by FastQC (version 0.11.5)