FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22692313
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT124700.010.40558894-95
AGCGTCG142450.09.95983994-95
CGTCGTG117600.09.74047694-95
TCGTGTA96100.08.97684494-95
GTCGTGT106200.08.88395794-95
GATCGGA392450.08.81087694-95
ATCGGAA363800.08.75351794-95
AGAGCGT201250.08.46688694-95
AGATCGG441950.08.41553494-95
AAGAGCG238750.08.39121194-95
CGTGTAG95750.08.38915794-95
TCGGAAG360450.08.294229594-95
GAGCGTC178850.08.2118394-95
CGGAAGA357850.07.92946294-95
TAGATCG135450.07.597125594-95
CAGATCG160650.07.026731594-95
AAGATCG155800.06.559057794-95
GTGTAGG134350.05.60740694-95
GAGATCG178900.05.48630894-95
GAAGAGC455800.05.255652494-95
Produced by FastQC (version 0.11.5)