FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22497547
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT99650.09.20773394-95
GCGTCGT113450.09.15629294-95
GATCGGA339200.09.1032494-95
CGTCGTG108750.09.09299194-95
AGCGTCG127700.09.08388594-95
ATCGGAA313050.09.0739694-95
TCGTGTA86250.08.57124794-95
AGAGCGT182300.08.18871494-95
GAGCGTC164250.08.14790294-95
AGATCGG381750.08.12595194-95
TCGGAAG314200.08.10262994-95
AAGAGCG217200.07.91263694-95
CGTGTAG88800.07.76296794-95
TAGATCG121850.07.74475594-95
CGGAAGA319750.07.20370594-95
AAGATCG138750.06.664373494-95
CAGATCG142600.06.18439394-95
GAGATCG157250.05.441950394-95
GTGTAGG126500.05.336672394-95
GAAGAGC430550.05.062772894-95