FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20853065
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG133300.09.79960194-95
GCGTCGT116400.09.75328294-95
ATCGGAA330550.09.29045994-95
TCGTGTA86700.09.23180594-95
GATCGGA360600.09.21441694-95
CGTCGTG108200.09.15344694-95
GTCGTGT98550.08.82064394-95
GAGCGTC170600.08.715075594-95
AGATCGG412050.08.64027494-95
AGAGCGT188950.08.54747594-95
TAGATCG122350.08.2501394-95
AAGAGCG221250.08.21209594-95
TCGGAAG328850.08.20457994-95
CGTGTAG88000.08.0158594-95
CGGAAGA333200.07.498710694-95
CAGATCG161900.06.557472794-95
AAGATCG138500.06.22490494-95
GAGATCG164400.05.90877394-95
GTGTAGG123350.05.295041694-95
GAAGAGC442900.05.024695494-95
Produced by FastQC (version 0.11.5)