FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24406995
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA433650.09.494394-95
GATCGGA466050.09.4354394-95
TAGATCG172200.09.07289794-95
AGATCGG528700.08.89668894-95
TCGGAAG415650.08.70012194-95
GCACACG200650.08.44913594-95
CGGAAGA411400.08.23016394-95
ACACGTC137500.07.85709394-95
CGTCTGA106400.07.85514694-95
CACACGT173700.07.641263594-95
ACGTCTG127850.07.35440794-95
AAGATCG183300.07.33174294-95
CACGTCT150050.06.7093994-95
AGAGCAC359000.06.620531694-95
GAGATCG201200.06.46704394-95
CAGATCG175950.06.450476694-95
AGCACAC300300.06.175169594-95
GAAGAGC521400.05.660480594-95
AAGAGCA482900.05.654495294-95
GAGCACA359400.05.57593394-95
Produced by FastQC (version 0.11.5)