FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23710584
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA427500.09.44759794-95
ACACGTC137600.09.43891594-95
GATCGGA463000.09.18475994-95
TCGGAAG415550.09.027904594-95
AGATCGG523100.08.81944594-95
TAGATCG168950.08.60094994-95
CGGAAGA407650.08.3408294-95
CACACGT176550.08.32484494-95
CGTCTGA100750.08.15425394-95
ACCGCGC53900.08.1047151
GCACACG206850.07.874477494-95
CACGTCT152000.07.73240994-95
ACGTCTG125700.07.574644694-95
CAGATCG178050.07.13452394-95
AGAGCAC355100.06.39234894-95
AAGATCG173800.06.37999494-95
AGCACAC304600.06.142560594-95
ACTTAAC67250.06.07218361
GAGATCG197900.06.046971394-95
ACTTTAC103150.05.80016041
Produced by FastQC (version 0.11.5)