FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21021139
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT97150.09.491611594-95
TCGTGTA84900.09.20956894-95
CGTCGTG106950.09.19963594-95
AGCGTCG128650.08.83017294-95
GCGTCGT111600.08.73113594-95
GAGCGTC162850.08.61024194-95
AGAGCGT181700.08.54101294-95
CGTGTAG86350.08.28428994-95
ATCGGAA307400.08.26465994-95
GATCGGA330450.08.24194994-95
AGATCGG374850.08.1216294-95
TCGGAAG311750.07.989248394-95
TAGATCG117100.07.854257694-95
AAGAGCG212000.07.835963294-95
CGGAAGA310150.07.61667894-95
CAGATCG143650.06.02208494-95
AAGATCG128850.06.01290394-95
GTGTAGG125000.05.646735794-95
GAAGAGC415900.05.171430694-95
GAGATCG154350.05.15809494-95
Produced by FastQC (version 0.11.5)