FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23663119
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT132200.010.19210794-95
GTCGTGT112500.010.18138694-95
CGTCGTG125050.09.67267194-95
AGCGTCG147500.09.50544394-95
ATCGGAA375800.09.25756394-95
TCGTGTA99000.09.24140494-95
GATCGGA408450.08.90735394-95
TCGGAAG369400.08.70388894-95
AGAGCGT210350.08.3147294-95
AGATCGG456950.08.29997294-95
TAGATCG134900.08.08561694-95
GAGCGTC193100.08.07298194-95
AAGAGCG249300.07.97839594-95
CGGAAGA370650.07.879528594-95
CGTGTAG101050.07.36072194-95
AAGATCG156500.06.63558994-95
CAGATCG169650.06.205291794-95
GAGATCG188900.05.497457594-95
GTGTAGG142450.05.3215894-95
GAAGAGC489700.05.168111394-95
Produced by FastQC (version 0.11.5)