FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19981826
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG135250.09.78034294-95
GTCGTGT103900.09.66854194-95
CGTCGTG110850.09.64079894-95
TCGTGTA93400.09.2044594-95
GCGTCGT119200.09.04514994-95
GATCGGA367450.08.86084994-95
ATCGGAA341450.08.79136194-95
AGATCGG416250.08.66641994-95
AAGAGCG220200.08.56326494-95
AGAGCGT189150.08.52510294-95
GAGCGTC169750.08.5200894-95
TAGATCG133550.08.26885194-95
CGTGTAG90300.08.12656294-95
CGGAAGA323050.07.990905394-95
TCGGAAG326350.07.931932494-95
CAGATCG149300.07.173859694-95
AAGATCG139100.06.982843494-95
GAGATCG151550.06.346513794-95
GTGTAGG120950.06.04757194-95
GAAGAGC414050.05.529175894-95
Produced by FastQC (version 0.11.5)