FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23178533
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG125900.09.19127394-95
GTCGTGT114800.09.18995894-95
TCGTGTA100150.08.92091394-95
GCGTCGT135700.08.72010994-95
AGCGTCG148400.08.56628194-95
ATCGGAA347650.08.49574294-95
GATCGGA375350.08.4638494-95
AGAGCGT208550.08.10087394-95
AGATCGG422650.07.88767994-95
GAGCGTC188200.07.840511394-95
TCGGAAG349050.07.80134494-95
CGTGTAG102450.07.76971894-95
AAGAGCG243900.07.40413394-95
TAGATCG124700.07.25990594-95
CGGAAGA356150.07.245515394-95
CAGATCG163500.05.66786294-95
AAGATCG144050.05.657874694-95
GAGATCG176800.05.496844394-95
GTGTAGG146100.05.33452994-95