FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21466388
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG122050.010.19839594-95
CGTCGTG100050.09.78179294-95
GCGTCGT106200.09.61794494-95
GAGCGTC158550.09.39376394-95
TCGTGTA82450.08.9023894-95
GTCGTGT92550.08.82917994-95
CGTGTAG81000.08.82713594-95
GATCGGA339950.08.72042894-95
AGATCGG381050.08.633882594-95
ATCGGAA315750.08.56125194-95
AGAGCGT174100.08.51381394-95
TAGATCG120500.08.27943194-95
AAGAGCG203250.08.04074394-95
CGGAAGA308600.07.982173494-95
TCGGAAG305600.07.943938394-95
AAGATCG131400.06.52604694-95
CAGATCG137800.06.48152194-95
GAGATCG145450.05.911982594-95
GTGTAGG124450.05.19173394-95
GAAGAGC400650.05.18777694-95
Produced by FastQC (version 0.11.5)