FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25284626
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA442350.09.68320594-95
ATCGGAA407350.09.58258694-95
AGATCGG500400.09.029633594-95
TCGGAAG397100.08.83139494-95
TAGATCG159700.08.66786894-95
ACACGTC130650.08.52338594-95
CGGAAGA394500.07.998832794-95
CACACGT170550.07.935454494-95
CGTCTGA101450.07.700031394-95
GCACACG199050.07.38375294-95
ACGTCTG126100.07.211611794-95
AAGATCG178850.07.0627194-95
CACGTCT143600.06.746124794-95
GAGATCG194000.06.731465394-95
CAGATCG171000.06.56770594-95
AGAGCAC350900.06.015434394-95
AGCACAC304100.05.809050694-95
GAAGAGC515400.05.60653994-95
GAGCACA357500.05.43282794-95
AAGAGCA476650.05.300180494-95
Produced by FastQC (version 0.11.5)