FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25284626
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2222240.8788898044210739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG154000.010.36410394-95
GCGTCGT133100.010.36195294-95
CGTCGTG126300.010.13585294-95
GATCGGA446550.09.48728194-95
ATCGGAA409750.09.36697294-95
TCGTGTA98350.09.20493394-95
CGTGTAG98100.09.01210194-95
AGATCGG504600.08.96117994-95
GTCGTGT117200.08.95150894-95
TCGGAAG396950.08.81377394-95
GAGCGTC195800.08.76561694-95
AAGAGCG263250.08.66007994-95
AGAGCGT221750.08.54783694-95
TAGATCG152550.08.31445694-95
CGGAAGA396450.07.96856694-95
AAGATCG182450.07.01648794-95
GAGATCG199400.06.644693494-95
CAGATCG180250.06.52663194-95
GTGTAGG144500.05.726681794-95
GAAGAGC508450.05.485298694-95
Produced by FastQC (version 0.11.5)