FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23119679
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT115450.09.40703494-95
CGTCGTG127150.09.25165294-95
GCGTCGT132200.09.2397994-95
AGCGTCG153250.09.13366894-95
TCGTGTA102700.09.0708194-95
ATCGGAA374350.08.77960294-95
AGAGCGT211750.08.64167194-95
GAGCGTC194650.08.52180394-95
GATCGGA400400.08.48142194-95
AAGAGCG254350.08.18470194-95
TCGGAAG373650.08.02647494-95
TAGATCG138650.07.918675494-95
AGATCGG450150.07.903054794-95
CGTGTAG102450.07.72436494-95
CGGAAGA371300.07.68685194-95
AAGATCG154500.06.567950294-95
CAGATCG166300.05.959011694-95
GAGATCG189650.05.43835294-95
GTGTAGG140900.05.36347894-95
GTAGGGA133350.05.061226494-95
Produced by FastQC (version 0.11.5)