FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21284863
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1856440.87218790179669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG122400.09.72619594-95
CGTCGTG101200.09.69049294-95
GCGTCGT108100.09.33364194-95
GATCGGA363150.09.29586994-95
ATCGGAA334900.09.17196394-95
AGAGCGT179400.09.13262194-95
AGATCGG411200.08.97210394-95
GAGCGTC158800.08.84766894-95
GTCGTGT96800.08.84024494-95
AAGAGCG213450.08.82436994-95
TCGGAAG328200.08.70556794-95
TAGATCG122850.08.52002494-95
TCGTGTA84400.08.43524194-95
CGGAAGA323200.08.16920394-95
CGTGTAG84400.07.709028294-95
ACCGCGC46650.07.3340541
CAGATCG152750.07.11463794-95
AAGATCG131150.06.90233494-95
GAGATCG171450.05.96740194-95
CGATCTA13601.8155387E-45.58968835
Produced by FastQC (version 0.11.5)