FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21096651
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA349450.08.88908994-95
TAGATCG128200.08.725039594-95
GATCGGA373900.08.701611594-95
AGATCGG424800.08.374557594-95
TCGGAAG347500.08.12571594-95
CGGAAGA344100.07.860924294-95
ACACGTC129850.07.58998194-95
CGTCTGA96600.07.32611494-95
ACGTCTG116500.07.03279194-95
CACGTCT134800.07.02938994-95
AAGATCG140950.07.009108594-95
CAGATCG156800.06.845842494-95
CACACGT159200.06.548713794-95
GCACACG186550.06.505192894-95
GAGATCG176450.05.94887994-95
AGAGCAC309550.05.81531194-95
AGCACAC266600.05.62978194-95
GAGCACA320050.05.179312794-95
GAAGAGC444650.05.13796794-95
AAGAGCA411150.05.111843694-95
Produced by FastQC (version 0.11.5)