FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23568046
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG143850.010.02786694-95
GCGTCGT126800.09.61452594-95
CGTCGTG118250.09.34504894-95
GTCGTGT110500.08.73159294-95
TCGTGTA93850.08.68539494-95
ATCGGAA351050.08.66504294-95
GAGCGTC182150.08.48035194-95
AAGAGCG238650.08.29494194-95
AGAGCGT200100.08.27780894-95
GATCGGA379250.08.27764894-95
AGATCGG424750.08.10148994-95
TCGGAAG352350.08.08001694-95
TAGATCG130850.07.918491494-95
CGTGTAG96950.07.843896494-95
CGGAAGA351000.07.197071694-95
CAGATCG161350.05.876701494-95
AAGATCG145450.05.783879394-95
GTGTAGG138000.05.665607594-95
GAGATCG185100.05.41795994-95
GAAGAGC471100.05.301750794-95
Produced by FastQC (version 0.11.5)