FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20476027
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG128850.09.14164494-95
AGCGTCG157400.09.11295494-95
GTCGTGT121700.08.85915594-95
TCGTGTA106500.08.6964594-95
GCGTCGT136100.08.20099894-95
GATCGGA377400.08.16140894-95
ATCGGAA350500.08.10345494-95
GAGCGTC188850.07.985162394-95
AGATCGG421150.07.73649694-95
AAGAGCG239200.07.644631494-95
CGTGTAG103400.07.418378494-95
AGAGCGT204100.07.17908694-95
CGGAAGA349400.07.048275594-95
TAGATCG134150.07.02775792-93
TCGGAAG339350.06.956095794-95
CAGATCG148700.06.036814794-95
AAGATCG141100.05.97486994-95
GAGATCG154900.05.687867694-95
GTGTAGG140850.05.5133894-95
TGTAGGG154850.05.045586694-95
Produced by FastQC (version 0.11.5)