FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21463290
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA416500.08.72922594-95
GATCGGA449900.08.7036294-95
ACACGTC142000.08.50670694-95
AGATCGG502800.08.37317894-95
TCGGAAG402950.08.28657294-95
CGGAAGA395200.08.02872394-95
TAGATCG160900.07.890958394-95
CACACGT176050.07.818508694-95
CACGTCT147650.07.779360394-95
GCACACG201650.07.732129694-95
CGTCTGA99700.07.712262694-95
ACGTCTG127050.07.58373994-95
AAGATCG168150.06.87328194-95
CAGATCG165750.06.285545394-95
AGAGCAC340350.06.27550694-95
GAGATCG178900.06.27455394-95
AGCACAC289450.06.247602594-95
GAGCACA339850.05.823857394-95
GAAGAGC487450.05.79360594-95
AAGAGCA455300.05.40521694-95
Produced by FastQC (version 0.11.5)