FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23486465
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA164000.010.94827594-95
GTCGTGT188850.010.3628294-95
CGTCGTG214050.010.163609594-95
AGCGTCG262000.010.08024894-95
GCGTCGT231100.010.05093994-95
CGTGTAG158650.09.7006994-95
GAGCGTC317900.09.41342694-95
AGAGCGT359800.09.03010294-95
ATCGGAA652650.08.77373794-95
AAGAGCG411500.08.71514994-95
GATCGGA713850.08.64037794-95
TCGGAAG631200.08.57145394-95
AGATCGG796250.08.53069394-95
CGGAAGA615100.08.30157494-95
TAGATCG228150.08.19260494-95
CAGATCG277500.06.86402994-95
AAGATCG243900.06.835858394-95
GTGTAGG190200.06.830374294-95
GAGATCG281700.06.635224394-95
GAAGAGC697950.05.941384394-95
Produced by FastQC (version 0.11.5)