FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19622069
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG109650.09.20509394-95
CGTCGTG89450.08.97396494-95
GCGTCGT93450.08.86939694-95
GTCGTGT85100.08.76290394-95
TCGTGTA75150.08.65903594-95
GATCGGA287550.08.52342794-95
AGATCGG323600.08.49127294-95
TAGATCG102150.08.39299194-95
ATCGGAA265150.08.32093394-95
GAGCGTC139350.08.0953394-95
AAGAGCG184850.08.04271894-95
CGGAAGA271500.07.636464694-95
AGAGCGT153800.07.628137694-95
TCGGAAG261900.07.51738894-95
CGTGTAG76700.07.121645594-95
AAGATCG109100.06.182178594-95
CAGATCG125400.06.098264794-95
GAGATCG127300.05.578156594-95
Produced by FastQC (version 0.11.5)