FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19613600
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG106900.010.2416794-95
GTCGTGT97300.09.86088994-95
TCGTGTA84600.09.82528694-95
GCGTCGT112350.09.57574794-95
ATCGGAA341700.09.56359294-95
AGCGTCG131600.09.51047794-95
GATCGGA375550.09.08101294-95
AGATCGG424050.08.74805594-95
GAGCGTC166750.08.67359294-95
TCGGAAG328000.08.63077894-95
CGGAAGA329150.08.44188794-95
AAGAGCG221100.08.41047694-95
TAGATCG135400.08.34902694-95
CGTGTAG83550.07.816886494-95
AGAGCGT184000.07.71846594-95
CAGATCG148050.06.592967594-95
AAGATCG146350.06.36122794-95
GAGATCG158700.06.120599394-95
GAAGAGC421450.05.544936794-95
GTGTAGG119200.05.25987294-95
Produced by FastQC (version 0.11.5)