FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23173280
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA402550.08.76470194-95
TCGGAAG395650.08.40146494-95
GATCGGA431500.08.39125994-95
AGATCGG485650.08.23297594-95
TAGATCG147700.07.90902194-95
ACACGTC145850.07.797712394-95
CGGAAGA389400.07.743653394-95
CGTCTGA109000.07.53681394-95
GCACACG215900.07.06025794-95
CACACGT183350.06.876250794-95
CACGTCT151850.06.848531294-95
ACGTCTG131100.06.845847694-95
CAGATCG161950.06.58269194-95
AAGATCG161450.06.50013494-95
AGAGCAC348900.06.03616194-95
GAGATCG201650.05.922538394-95
AGCACAC308400.05.40456794-95
GAGCACA353000.05.37415594-95
GAAGAGC505700.05.14113894-95
AAGAGCA456950.05.04531994-95
Produced by FastQC (version 0.11.5)