FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20529479
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA411600.08.91791794-95
CGTCTGA103600.08.72033294-95
GATCGGA444200.08.63709694-95
CACACGT179050.08.52863294-95
TCGGAAG398950.08.3923694-95
AGATCGG499850.08.235270594-95
TAGATCG163700.08.22749294-95
CGGAAGA390400.08.09025694-95
ACACGTC139550.08.03709594-95
ACGTCTG123200.07.794928694-95
GCACACG203100.07.670492694-95
CACGTCT140550.06.866436594-95
AGAGCAC337800.06.74577194-95
CAGATCG173950.06.720325594-95
AAGATCG157450.06.34026394-95
GAGATCG176150.06.12486694-95
ACCGCGC43250.06.03437141
GAGCACA339250.05.850236494-95
AGCACAC288250.05.84059294-95
AAGAGCA443400.05.75419394-95
Produced by FastQC (version 0.11.5)