FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20529479
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG134050.010.32838294-95
GCGTCGT143600.09.65803994-95
AGCGTCG165150.09.26057294-95
GAGCGTC204650.08.8076694-95
ATCGGAA403450.08.78232994-95
GTCGTGT122600.08.65859894-95
TCGTGTA105800.08.64182894-95
AGAGCGT226600.08.62522694-95
CGTGTAG106500.08.60732694-95
AAGAGCG265550.08.52269894-95
GATCGGA435250.08.48987694-95
TAGATCG152250.08.31381794-95
AGATCGG489200.08.20895294-95
TCGGAAG385800.07.99608694-95
CGGAAGA386850.07.91299494-95
AAGATCG149800.06.57910594-95
CAGATCG175600.06.38334194-95
GTGTAGG145200.06.37864694-95
GAGATCG177350.05.7847394-95
GAAGAGC469500.05.467900894-95
Produced by FastQC (version 0.11.5)