FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23851882
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG157100.09.81123894-95
CGTCGTG126450.09.55991994-95
TCGTGTA104350.09.49071494-95
ATCGGAA415350.09.34886494-95
GATCGGA451250.09.19982494-95
AAGAGCG269050.09.11848494-95
GTCGTGT118550.09.11517794-95
GCGTCGT134950.09.0457794-95
GAGCGTC201250.08.85076294-95
AGAGCGT225050.08.716784594-95
AGATCGG506750.08.6187394-95
CGTGTAG108150.08.60824994-95
TAGATCG160900.08.23632494-95
TCGGAAG391700.08.22775994-95
CGGAAGA401150.08.134582594-95
AAGATCG171350.06.97170894-95
CAGATCG175800.06.592592794-95
GTGTAGG146300.06.120012894-95
GAGATCG191350.05.9203294-95
GAAGAGC504800.05.405750394-95
Produced by FastQC (version 0.11.5)