FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18702089
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT31750.034.262829
TCCGATC35000.032.3046232
TTCCGAT42400.027.0026861
CCGATCT41550.026.9831873
CTCCGCG52450.021.9162588
ATCTCCG56200.020.030046
CGATCTC64300.018.1012024
TCTCCGC75600.015.0784187
CCGCGTA11400.011.87603310-11
CGCGTAA5551.906301E-98.55930210-11
ACGCGGA63550.07.924823894-95
GATCTCC153250.07.87373545
CCGCGTT19000.07.50065210-11
TACGCGG23450.06.98997494-95
CGCGGAG74650.06.20546294-95
TAACGCG6956.093578E-66.152576494-95
AGATCGG43150.06.00088194-95
ATACGCG7159.649853E-65.98047694-95
CGGAGAT66650.05.95231194-95
AACGCGG28550.05.82453594-95
Produced by FastQC (version 0.11.5)