FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004721979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004721979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15196121
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC31300.027.1689155
TTCCGAT36000.023.6227194
CCGATCT38200.022.3857356
CGATCTA11650.020.3849247
CTTCCGA49900.017.1434633
TCTTCCG69000.012.6045862
CGATCTG38850.012.4735054
CGATCTT31300.07.7391067
CAGATCG61400.07.43429394-95
AGATCGG48750.06.9737894-95
GATCGGA39400.06.878843394-95
ATCGGAA42400.06.44820594-95
GAAGTCG43500.06.01665541
GAAGTCT119700.05.7644191
TCGGAAG59200.05.421492694-95
GACTTCA194250.05.128139594-95
Produced by FastQC (version 0.11.5)