FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004722090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004722090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19266985
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAAGT163800.032.9186331
CGATCTA18600.032.437494
ATCAAGC118050.023.4618571
ATCAAGG168200.023.245221
ATCAAGA198050.022.9320491
TCCGATC28800.021.2792852
TTCCGAT36250.017.1681331
CCGATCT37850.016.8188133
TCAAGTG147700.012.5442282
TCAAGTA108750.012.0133262
GATCTAT54150.011.8437575
TCAAGTC120000.010.609952
TCAAGAG155750.010.3707622
TCAAGGG128800.010.0325842
ATCTATC68700.09.4027566
TCAAGTT142100.08.7926852
CTATCAA77350.08.4744618
TCAAGCG36400.08.0918922
TAGATCG29100.07.918860494-95
TCAAGCA146100.07.73903042
Produced by FastQC (version 0.11.5)