FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004722282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004722282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20319170
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC39750.030.7144812
TTCCGAT47950.025.858251
CCGATCT49700.024.8520983
CGATCTT50300.024.6500994
ATCTTGT144100.09.1633956
GATCTTG153500.08.356085
TTGTTCA176700.07.581329
CTTGTTC188850.07.1182968
TCTTGTT219700.06.18306887
CTTCCGA63450.06.06457852
AGATCGG42550.05.75052994-95
AAGATCG50850.05.65281294-95
GATCGGA34250.05.41008694-95
TGTTCAA150700.05.26441861
TGTTCAT192350.05.1618021
Produced by FastQC (version 0.11.5)