FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004722430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004722430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20359092
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA32900.055.3040164
TCCGATC43950.041.615862
TTCCGAT50250.036.6819731
CCGATCT53550.034.155063
GATCTAG66050.028.410315
ATCTAGG78750.023.6433686
TAGGTTC82450.022.7006939
CTAGGTT83450.022.3699828
AGGTTCG41900.020.9756151
TCTAGGT107250.018.0245537
GGTTCGA24550.09.6755472
AGGTTCC144700.09.5539171
TAGATCG30500.09.42450294-95
AGGTTCA150300.09.1979491
AGGTTCT188900.08.2741041
GGTTCGT25900.08.0706772
GGTTCGC23900.07.9509522
GGTTCGG35700.07.8512872
AGATCGG44000.06.532893794-95
GGTTCTA62650.05.99049952
Produced by FastQC (version 0.11.5)