FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004723123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45713067
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1924300.4209518473131545TruSeq Adapter, Index 9 (100% over 50bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1222770.2674880685647279Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA789040.17260710159744916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)