FastQCFastQC Report
Wed 11 May 2022
EGAF00004723280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23125218
Sequences flagged as poor quality0
Sequence length8-383
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC4681202.024283619726309No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG4623301.9992460179186204No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG4374741.8917616257714847No Hit
GAGCATGGTAATGGATTTATGGTGGGTCCTTCTCTGTGGGCCTCTCATAG2870031.241082354337157No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2718251.1754483784758267No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC2274800.9836880240437085No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG2016010.8717798898155252No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC1321570.5714843423313891No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA1157510.5005401462593779No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA1050020.45405842228168397No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC1022040.44195907688308067No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA1019690.44094287024667184No Hit
CGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGA900270.38930227598286854No Hit
AGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG828220.358145812938931No Hit
TGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA779140.33692222923044446No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT774700.3350022473301657No Hit
TATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC733510.3171905233498772No Hit
AGAGTACACACAAGAAGAAAAGTAACAGCACTAGATTGTAAAGACTGGGG717770.3103841010277179No Hit
GGAGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT668100.2889053845892393No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC651790.28185247810420644No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA633010.2737314735800545No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC601360.2600451161152297No Hit
CCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTCTG589500.25491651581403474No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA581130.25129709047499577No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC555410.24017503316076846No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA528030.2283351447757163No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAG504460.21814280842671405No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA487150.21065747358576253No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC446890.19324790797647834No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC436160.18860795171747138No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC385750.16680923829561306No Hit
GACCTCCTGGGATCGCATCTGGAGAGTGCCTAGTATTCTGCCAGCTTCGG378950.16386872547536632No Hit
GGCAGATGATGTTTGTTTTCACGATGGTCTTCAGATGCCCACGTGGGCAC376530.16282224885404323No Hit
AGAAGAAAAGTAACAGCACTAGATTGTAAAGACTGGGGTGGACCTCTTTC362620.15680717042321504No Hit
GTAGGGTGATGAAAAAGAATCCTTAGGCGTGGTTGTGGCCGTCTTGGTCA360230.1557736666525695No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGGAGTCCAGGAGTTCTGGGCTGT337220.14582349018288174No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT332620.14383431974565603No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC321710.13911652638258373No Hit
AAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTG308260.1333003649954781No Hit
GTCTAATGATGAATTTCATAGGGCAGATTCTGAGGTGAAAATTTAATTCA300250.12983661386456985No Hit
GGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTT289190.12505395624810975No Hit
TCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAAA277230.11988211311132288No Hit
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGTTC273490.1182648310601872No Hit
GTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAG270710.11706268023073339No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG270450.11695024885819455No Hit
GATCGATGATGAGTCCCCCATAAAAACATTCCTTGGAAAAGCTGAACAAA253200.10949085971859811No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC252650.10925302412284287No Hit
AACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCT248430.10742817646086622No Hit
AGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAGTG243240.10518387329364852No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC241360.10437090798452149No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGTGTCTGGAACTCTGAGGT238520.10314281145371257No Hit
TAGGGTGATGAAAAAGAATCCTTAGGCGTGGTTGTGGCCGTCTTGGTCAC236700.10235579184594064No Hit
GAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTG232360.10047905278125378No Hit
ACACCCAACAGGAATCTGGGGTCATTGTGATGAGGGCGTCAAACTTGTGG232250.10043148566210272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)