FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24338178
Sequences flagged as poor quality0
Sequence length8-383
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG4126111.695324111772048No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC3950001.6229645456615527No Hit
GAGCATGGTAATGGATTTATGGTGGGTCCTTCTCTGTGGGCCTCTCATAG3169751.3023776882558753No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG2208200.9072988125898332No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT1804410.7413907483132057No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG1626980.6684888244304894No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA1416980.5822046333953182No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC1306710.53689721555985No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC1256570.5162958377574525No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC1121510.46080277660883245No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC1110300.4561968443159549No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC1052820.4325796285983281No Hit
TAGATTGAGGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT984280.40441811215284895No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG930840.3824608399198987No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA913630.37538964502601635No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA777710.3195432295712522No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT744400.3058569133646734No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGTGTCTGGAACTCTGAGGT703180.28892055929576976No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGC641450.2635571159024312No Hit
GAGAGTGATGAGTTGCACACTGGTGGAGCCATGGTATCAGGTGATACAGG635330.2610425480494062No Hit
GGCAGATGATGTTTGTTTTCACGATGGTCTTCAGATGCCCACGTGGGCAC630400.25901692394558046No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGCCATGTCACCCTTCTGACT617410.2536796304144049No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA599250.24621810227536345No Hit
GTCTAATGATGAATTTCATAGGGCAGATTCTGAGGTGAAAATTTAATTCA585310.24049047549902872No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC575290.23637348695535057No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC551720.22668911370440303No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA551470.22658639442936115No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC536600.2204766519498707No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA532130.21864003131212206No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA526760.21643362128422267No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC464650.19091404459282038No Hit
GACCTCCTGGGATCGCATCTGGAGAGTGCCTAGTATTCTGCCAGCTTCGG453020.18613554391787257No Hit
AGAGTACACACAAGAAGAAAAGTAACAGCACTAGATTGTAAAGACTGGGG409690.16833223916761558No Hit
AGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC404690.1662778536667782No Hit
AGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG404250.1660970677427045No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCT375590.15432133005190446No Hit
TGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA374900.1540378248527889No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC363150.149210018925821No Hit
TTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCT354010.14545460223029022No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAG348010.1429893396292853No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC336840.13839984242041453No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATGGCTTTCTCACCA317430.13042471790616372No Hit
GTGCTGTGATGATGCCTTAATATTGTGGTTTCGACTCACTGAGAGTAAAA312560.12842374642834808No Hit
GAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTG309870.12731848702889756No Hit
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGTTC309060.1269856765777619No Hit
ACACCCAACAGGAATCTGGGGTCATTGTGATGAGGGCGTCAAACTTGTGG301580.12391231586850913No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGTTATACCCTTCCCGTACTACCA299260.12295908099612057No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGGAGTCCAGGAGTTCTGGGCTGT297880.12239207059788945No Hit
GACCAATGATGAGACAGTGTTTATGAACAAAAGATCATGATTAATCCAGTTCTGCACAAAACACTGAGGT297750.12233865657486767No Hit
TAGATTGATGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT289850.11909272748354457No Hit
TTGCAATGATGTCGTAATTTGCGTCTTACTCTGTTCTCAGCGACAGTTGC282910.11624124040838227No Hit
GTAGGGTGATGAAAAAGAATCCTTAGGCGTGGTTGTGGCCGTCTTGGTCA282100.1159084299572466No Hit
AGAAGAAAAGTAACAGCACTAGATTGTAAAGACTGGGGTGGACCTCTTTC280320.11517706871894846No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG279990.1150414792758932No Hit
TAGATTGAAGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT279690.11491821614584297No Hit
GACCAATGATGAGTACCATGGGGTATCTGAAACAGGATTTTTGATTAAACCCATATGCAATTCTGAGGT275520.11320485863814457No Hit
GGAGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT262310.10777717214493213No Hit
GAGGAATGATGACAAGAAAAGGCCGAATTGCAGTGTCTCCATCAGCAGTT259500.10662260749346152No Hit
GACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGATTAAAGCCATCTGTAACTCTGAGGT256320.10531601831492891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA14750.0135038.7360-363
GTACGTC11156.476361E-629773.105340-349
CTACGTA9056.184564E-1127511.338350-359
AGCTACG21201.8444553E-923488.451350-359
ACGTACG8705.2750693E-1119078.742350-359
GATGTAC53151.2631954E-618737.73360-363
TACGTAC13150.015146.927350-359
TCGATCG11101.1510565E-814953.609350-359
GCTACGT22302.2591848E-914886.553350-359
AGCATCG36101.2031087E-613793.772350-359
GAGCATC76251.133027E-513061.12350-359
TACGTCT19354.3293483E-512867.06340-349
ATCGATC17454.4719854E-811414.445350-359
CATCGAT27158.45186E-69170.445350-359
ATGTACA77251.8241417E-58594.696360-363
TGTACAG86602.877409E-55750.06360-363
CCCTAAA53602.4913187E-45308.691310-319
ACGGTAC7902.9068987E-45252.692290-299
ACGAATC9754.4276426E-44256.0273300-309
ACGTCTG47952.8188479E-64153.9536340-349
Produced by FastQC (version 0.11.5)