FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004723282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39779916
Sequences flagged as poor quality0
Sequence length8-384
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG7257301.8243628267088345No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA6915361.7384048774763627No Hit
CGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAA6349931.5962653113696872No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC4264331.0719806447052327No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG3575900.8989209529753658No Hit
ATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC3241960.8149740688240769No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC2617340.6579551349479974No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA2023050.5085606515609535No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG1772760.44564196666478634No Hit
ACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCG1691990.42533775083889064No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA1566190.3937137524372852No Hit
TTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA1529430.38447290838924847No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAAC1505190.3783793811932635No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC1432450.3600937719426054No Hit
AGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAA1386260.348482384930124No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG1312730.3299981830026992No Hit
CGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGC1284150.3228136530001723No Hit
GATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG1170740.2943042916430492No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTT1167670.2935325454181452No Hit
TGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATC1158470.2912198205747845No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC1093140.2747969603555724No Hit
GATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAG1031030.2591835538315365No Hit
TCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTT1009210.2536983738226094No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC1004410.25249173477389947No Hit
AGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAA982810.24706185905470487No Hit
AGACGGACCAGAGCGAAAAGCATTTGCCAAGAATGTTTTCATTAATCAAG967600.24323832156910538No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA929010.23353744638374802No Hit
GTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCT914950.23000299950356856No Hit
CGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGA899000.22599343849795964No Hit
GATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATG896610.22539263280495614No Hit
GGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAA870800.21890443408678892No Hit
AGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAA844160.21220758736644896No Hit
TGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCA787240.19789885931383064No Hit
GACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCAGGAC787160.1978787486630188No Hit
AGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAA781100.19635536686402255No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA766580.1927052837416751No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTG756240.19010598212424581No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC723610.18190335042436992No Hit
GGATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCAT683790.17189327398278065No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC679150.17072685623569442No Hit
GGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGG676060.1699500823480874No Hit
GAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTG672870.16914817014696562No Hit
GGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTG663740.16685304212306531No Hit
ACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTT647060.16265997142879837No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT646680.16256444583744217No Hit
CGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTAT643240.1616996878525334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCA635860.15984448031514192No Hit
GATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATG620660.1560234566608939No Hit
AAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTG592280.14888920328539657No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA578230.1453572702365686No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA566890.14250658548399148No Hit
ACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACT562410.1413803890385289No Hit
AGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGA531330.13356740119813224No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACCA509910.12818277444326429No Hit
CACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT509890.12817774678056132No Hit
CGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAG501960.1261842785188385No Hit
CGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCC500150.1257292750442208No Hit
AGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAA492590.1238288185425027No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCC477600.12006058534663573No Hit
CAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGAT475920.11963826167958726No Hit
GATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCATA473540.11903996981793526No Hit
AGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGG470680.11832101405141228No Hit
CACGCCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC463600.11654122145456516No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT460390.11573428159074041No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC459130.11541753884045407No Hit
TGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA453850.11409023588687317No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA441060.11087504558833156No Hit
AGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAAC434470.10921843072770691No Hit
TCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGC423650.10649846520540668No Hit
TACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCG409650.10297910131333611No Hit
TATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC407170.10235567113816932No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC406520.1021922721003232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)