FastQCFastQC Report
Wed 11 May 2022
EGAF00004723283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25920161
Sequences flagged as poor quality0
Sequence length8-384
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG5249582.025288346009888No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC4823791.8610185330253155No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG3389381.30762305064386No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC3093711.1935535431280693No Hit
GAGCATGGTAATGGATTTATGGTGGGTCCTTCTCTGTGGGCCTCTCATAG2151950.8302224665965616No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG2091240.8068005441787186No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC1936210.7469899588972461No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC1709010.6593361823639907No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG1626930.6276697123910612No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA1082580.41765944277892414No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1080430.41682997262246946No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA989570.3817761780106227No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT911610.3516992043375039No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT844620.3258544574626678No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA836720.3228066368877879No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA791740.3054533496146108No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC785720.3031308331765378No Hit
TGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA700620.270299246983844No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA612670.23636813058375677No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC578180.22306188607393293No Hit
AGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG575210.2219160598578072No Hit
TATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC552490.2131506822044817No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC538880.20789994321408728No Hit
TAGATTGAGGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT502090.19370635853689333No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA490010.18904589365783647No Hit
AAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTG465030.1794086078400516No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA464160.17907296177674206No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC463740.1789109257461788No Hit
GAGAGTGATGAGTTGCACACTGGTGGAGCCATGGTATCAGGTGATACAGG455910.17589011117639278No Hit
GATCGATGATGAGTCCCCCATAAAAACATTCCTTGGAAAAGCTGAACAAA429120.16555452722689493No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC416940.16085548234056107No Hit
TGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA404860.1561950174615042No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC399800.15424286909328996No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGGAGTCCAGGAGTTCTGGGCTGT395420.1525530647745591No Hit
GGCAGATGATGTTTGTTTTCACGATGGTCTTCAGATGCCCACGTGGGCAC394600.152236708714888No Hit
CGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGA394010.15200908667195392No Hit
TCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAAA356870.13768047196929062No Hit
GGAGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT352890.13614498767966757No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAG337360.13015351254955554No Hit
TCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGC334890.1292005863698146No Hit
GTCTAATGATGAATTTCATAGGGCAGATTCTGAGGTGAAAATTTAATTCA328810.12685492192737538No Hit
GTAGGGTGATGAAAAAGAATCCTTAGGCGTGGTTGTGGCCGTCTTGGTCA292870.11298926731203561No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC287510.11092137892199048No Hit
GGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGAGTCTCACGGCCAT265430.1024029133152375No Hit
GAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTG263130.10151557314786741No Hit
GACCTCCTGGGATCGCATCTGGAGAGTGCCTAGTATTCTGCCAGCTTCGG263060.10148856714277353No Hit
TGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA261790.10099860105035614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)