FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22240051
Sequences flagged as poor quality0
Sequence length8-384
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC4383571.9710251563721686No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG4009191.8026892114590922No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC2814691.26559511936371No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG2439571.0969264414006963No Hit
GAGCATGGTAATGGATTTATGGTGGGTCCTTCTCTGTGGGCCTCTCATAG2199360.9889185955553789No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG2149550.9665220641805182No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT1848190.8310187777896731No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC1325830.5961452156741907No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC1173590.527692135238359No Hit
GAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTG987110.44384340665405847No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC964120.4335062001431561No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA839530.3774856451543209No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA770010.34622672403044397No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG615600.2767979264076328No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG532550.239455386140976No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT527460.23716672232451264No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA512360.23037716954875687No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAG490960.22075488945596392No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG482570.21698241609248106No Hit
TAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCC468380.21060203504029731No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC455050.204608343748852No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA453220.20378550390914124No Hit
TGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA448610.2017126669358807No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA445200.20017939707062724No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC443550.1994374922971175No Hit
AGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTG420760.18919021363754965No Hit
GGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG419910.18880802026937799No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGG399130.179464516515722No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG397720.1788305251638137No Hit
AGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG377850.16989619313372978No Hit
GGATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCAT375380.16878558416974854No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC369210.1660113099560788No Hit
AAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCTGTGAATAGCCACTGCACTCC368610.16574152640207526No Hit
TCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGC360740.16220286545206214No Hit
GGAGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT335500.15085397061364653No Hit
AAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTG330590.14864624186338424No Hit
AGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCG330500.14860577433028369No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGGAGTCCAGGAGTTCTGGGCTGT318660.1432820455312805No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT318230.14308870065091128No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA303670.1365419530737587No Hit
GGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTT296250.13320562978924821No Hit
CGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGA293520.13197811461853212No Hit
GAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTG292450.13149700061389247No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA292400.13147451865105883No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGGAGTTCTGGGCTGTAG290760.13073711027011584No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT279460.12565618666971584No Hit
AGAAGAAAAGTAACAGCACTAGATTGTAAAGACTGGGGTGGACCTCTTTC278810.12536392115287864No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGT275730.12397903224232715No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC268490.12072364402401774No Hit
AGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAGTG263720.1185788647696896No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT251100.1129044173504818No Hit
TGGAGAGGAACAACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTT250050.1124322961309756No Hit
TGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA244550.10995928021927648No Hit
AGAGTACACACAAGAAGAAAAGTAACAGCACTAGATTGTAAAGACTGGGG243610.10953661931800426No Hit
GAGAGTGATGAGTTGCACACTGGTGGAGCCATGGTATCAGGTGATACAGG239320.10760766690687895No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT238710.10733338696030867No Hit
AGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC233700.10508069428437912No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC225820.10153753694179928No Hit
TGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTC225530.10140714155736424No Hit
AGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTG225490.10138915598709734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA7753.4769164E-644310.34330-339
GTACGTC11701.372373E-744026.297340-349
CTAACGT16903.30671E-640639.664330-339
CATCGAT26003.1170239E-626415.78320-329
AACGTAC9901.3616549E-523124.924330-339
TACGTCT15051.1072465E-522817.615340-349
ATCGATC15403.846726E-722299.035320-329
TCGATCG10651.5757783E-521496.408320-329
ACGTACG8553.6379788E-1220082.17330-339
CGATGTA14751.2163582E-811640.852320-329
AGATCAT91703.245208E-511234.628350-353
CTACGTA9357.0043825E-811018.347330-339
TGTACAG77505.2555806E-68862.068330-339
TACGTAC11251.2200508E-78324.973330-339
CGTACGT153200.06724.6436340-349
GATCATC82201.5645004E-46266.517350-353
ACGTCTG41451.856419E-46213.6035340-349
GTACAGC56251.461467E-66104.9805330-339
AGCTACG21757.2106013E-75920.7783330-339
AAAAAAC174951.1812227E-45888.6274350-353
Produced by FastQC (version 0.11.5)