FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004723285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24440683
Sequences flagged as poor quality0
Sequence length8-381
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG4061621.6618275356707501No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC3742331.5311887969742908No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG3545971.450847343341428No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC2739491.1208729314152146No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG2429670.994108879854135No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC2391340.9784260120717576No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA1975260.8081852704361822No Hit
GAGCATGGTAATGGATTTATGGTGGGTCCTTCTCTGTGGGCCTCTCATAG1802590.7375366719497978No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG1670980.6836879313069933No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC1078600.441313362642116No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA959890.3927427069038946No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG938230.3838804341106179No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC859020.3514713561810036No Hit
CGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGA817650.3345446606381663No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA793400.32462267932528727No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA655680.26827400854550587No Hit
TATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC551080.22547651389284007No Hit
GATCGATGATGAGTCCCCCATAAAAACATTCCTTGGAAAAGCTGAACAAA550300.22515737387535364No Hit
TGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA531160.21732616883087924No Hit
TCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAAA519120.21239995625326838No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA462400.18919274882784576No Hit
AAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTG450540.18434018394657792No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT435400.17814559437639282No Hit
AGAGTACACACAAGAAGAAAAGTAACAGCACTAGATTGTAAAGACTGGGG404670.1655722959951651No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT401920.16444712285659122No Hit
GACCTCCTGGGATCGCATCTGGAGAGTGCCTAGTATTCTGCCAGCTTCGG367900.1505277082477605No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA366780.15006945591495952No Hit
AGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG359300.14700898497803846No Hit
TAGATTGAGGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT355800.1455769464380353No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC352480.14421855559437516No Hit
GGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTT350020.14321203707768723No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC328980.1346034396829254No Hit
GGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGAGTCTCACGGCCAT325200.133056838059722No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC324940.1329504580538932No Hit
GAGAGTGATGAGTTGCACACTGGTGGAGCCATGGTATCAGGTGATACAGG321230.13143249720148983No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA309980.12682951618005112No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC303490.12417410757301667No Hit
GAGGAATGATGACAAGAAAAGGCCGAATTGCAGTGTCTCCATCAGCAGTT301320.12328624367821472No Hit
ACACCCAACAGGAATCTGGGGTCATTGTGATGAGGGCGTCAAACTTGTGG299860.1226888790300991No Hit
TCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGC285800.11693617563797215No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC282540.11560233402642635No Hit
GGAGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT268340.10979234909269925No Hit
GGCAGATGATGTTTGTTTTCACGATGGTCTTCAGATGCCCACGTGGGCAC266980.10923589983144089No Hit
GGTTCCATTCCGGTGCAATAGCATTTCCAGCGACACATGACTGACTGACT266100.10887584442709722No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAG258540.10578264118069042No Hit
TAGGGTGATGAAAAAGAATCCTTAGGCGTGGTTGTGGCCGTCTTGGTCAC255830.10467383419685938No Hit
TGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACC248950.10185885558108175No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC245280.1003572608834213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)