FastQCFastQC Report
Thu 12 May 2022
EGAF00004723286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41637104
Sequences flagged as poor quality0
Sequence length8-371
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGA4450121.0687871087287915No Hit
GGATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCAT4093190.9830630871926155No Hit
TGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGA2954230.7095186062892367No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC2913200.6996644147008879No Hit
ACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCG2236750.5372011463621486No Hit
GATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCATA2137990.5134819174743758No Hit
ATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC1364600.3277365303792502No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATGGCTTTCTCACCA1293900.3107564829676915No Hit
TTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA1118220.26856334676878585No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG1101690.26459333002602675No Hit
TGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG1078120.2589325136541677No Hit
CGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCC1062250.25512100937663673No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1030870.2475844621662448No Hit
GTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCT952310.2287166753960602No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG932240.22389645543071393No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC869710.20887860020235796No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC852750.2048053101867988No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGTGTCTGGAACTCTGAGGT844630.20285512652368906No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC789750.18967457486956826No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA788200.18930231074668402No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGG738650.1774018673344813No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC736290.1768350651860898No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA730990.17556216205622754No Hit
AGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGACCT682150.16383223962934598No Hit
GGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCC666240.16001112853574062No Hit
TGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC643700.15459768767779816No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGG626360.15043313290953186No Hit
AGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAA600480.14421752290937428No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA588070.14123700822228175No Hit
AAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTG585290.1405693345051087No Hit
GGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCC583450.14012742096568484No Hit
CGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGC578580.13895779110862275No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC573450.1377257169470768No Hit
GAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCC547580.1315125086509379No Hit
AGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAA541910.13015074247238712No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTG535780.1286784979089804No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG535700.12865928427683154No Hit
TGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCATAAT528630.12696127953567568No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGTGTCTGGAACTCTGAGGTC525010.12609186268093958No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGC495580.11902364775417619No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA491290.11799331673019334No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGTTACACCCTTCCCGTACTACCA488510.1173256430130203No Hit
AAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCC487770.11714791691564333No Hit
CGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAA485920.11670360167220083No Hit
GATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATG478470.11491433217833787No Hit
AAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATGGCTTTCTCACCA475890.11429469254153699No Hit
GATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG466600.11206350950825014No Hit
GAGCATGGTAATGGATTTATGGTGGGTCCTTCTCTGTGGGCCTCTCATAG463480.11131417785444445No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAAC452270.10862186764958486No Hit
GGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCC443240.10645312892078182No Hit
GAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTG442730.1063306420158328No Hit
ACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTT433540.10412347602273203No Hit
TAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCC424980.10206761738280357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)