FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21620657
Sequences flagged as poor quality0
Sequence length8-383
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG1975000.9134782536904406No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC1924610.890171838903878No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC1653430.7647454931642457No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG1329670.6149998124478826No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG1012870.46847327534958816No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC982950.4546346579569714No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC832430.38501605200989036No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC807230.3733605320134351No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA534670.24729590779780652No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA533710.24685188798841776No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT530390.24531631948094823No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC506530.23428057713509817No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATGGCTTTCTCACCA499420.2309920554218126No Hit
AAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATGGCTTTCTCACCA430890.19929551632034123No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC429160.1984953556221719No Hit
GGTCGATGATGAGAACCTTATATTGTCCTGAAGAGAGGTGATGACTTAAA391920.1812710871829658No Hit
TGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA352240.16291826839489662No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC345690.1598887582370878No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGC344700.15943086280865562No Hit
AAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCTGTGAATAGCCACTGCACTCC329250.1522849190013051No Hit
ACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCG327160.15131825087461498No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT324080.14989368731949265No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA317890.14703068459020463No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGTATACAACCCCCC315870.1460963929079491No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA304570.14086990973493543No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC297980.13782189875173545No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGTGTCTGGAACTCTGAGGT294880.1363880847839175No Hit
CGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAA292500.1352872856731412No Hit
TAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCC269540.12466781189859309No Hit
TCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGC251020.11610192974246805No Hit
AGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC244450.11306316917196364No Hit
GGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTT241280.11159697875971113No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGG241210.11156460231527654No Hit
AGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGC232280.1074342930466914No Hit
TAGATTGAGGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT227690.10531132333305136No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC225710.104395532476187No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC223620.10342886434949686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA7053.2455318E-7112340.77350-352
CGATGTA14301.3353001E-655384.785340-349
ACGTACG6602.8444028E-648000.15350-352
TACGTAC11200.035357.254350-352
GTACGTC10201.0190395E-525882.434320-329
TCGATCG7752.588452E-517032.31330-339
ATCGATC11754.0567476E-513480.893330-339
AGCATCG33104.2924177E-511963.783330-339
GAGCATC66902.9225312E-511838.602330-339
GATGTAC36705.276876E-510790.224340-349
TACGTCT18456.223539E-510731.74320-329
CATCGAT22209.0104506E-58918.946330-339
CCGGGGG115908.771498E-56833.4985330-339
GTCGAGA35250.06419.473300-309
GCTACGT16053.1956393E-44934.595340-349
AGCTACG19003.6062015E-44631.5933340-349
ATGTACA62153.7829884E-44247.801340-349
CGAGACG21054.4263012E-44180.5356300-309
TCATGAG60855.07682E-43718.7578300-309
TTTTCCC228900.03460.037350-352
Produced by FastQC (version 0.11.5)